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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-65539 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITCH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GFTGASQNDDG SRSKDETRVS TNGSDDPEDA GAGENRRVSG NNSPSLSNGG FKPSRPPRPS RPPPPTPRRP ASVNGS Highest antigen sequence identity to the following orthologs - mouse 78%, rat 82%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references IL-33 enhances Jagged1 mediated NOTCH1 intracellular domain (NICD) deubiquitination and pathological angiogenesis in proliferative retinopathy.
Sharma D, Bisen S, Kaur G, Van Buren EC, Rao GN, Singh NK
Communications biology 2022 May 19;5(1):479
Communications biology 2022 May 19;5(1):479
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ITCH in human cell line A-431 shows localization to nucleoplasm. Samples were probed using an ITCH Polyclonal Antibody (Product # PA5-65539).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ITCH was performed on HeLa cell lysates. Antibody-bead conjugates were prepared by adding 2 µg of ITCH polyclonal antibody (Product # PA5-65539) with 30 µL of protein G-Sepharose beads and rocked overnight at 4°C. 750 µg of lysate was incubated with an antibody-bead conjugate for 2 hours at 4°C. Following centrifugation and multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
