Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP0292 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#AP0292, RRID:AB_2797474
- Product name
- PKC IJ (phospho T538) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human PKC IJ around the phosphorylation site of Threonine 538.
- Description
- Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types. PKC theta is a member of the PKC family, and its predominant expression in hematopoietic cells suggests that it may play a role in signal transduction and growth regulatory pathways unique to these cells. PKC theta is a Ca(2+)-independent PKC isoform that is selectively expressed in T lymphocytes (and muscle), and it is thought to play an important role in T cell receptor-induced activation. Among several PKC isoenzymes expressed in T cells, PKC theta is unique in being rapidly recruited to the site of TCR clustering. It has been shown that PKC theta is essential for TCR-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of p-PKC ¦È (T538) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate