- 700043 - Invitrogen Antibodies PRKCQ antibody

Submitted references PD-1 is a haploinsufficient suppressor of T cell lymphomagenesis.

Wartewig T, Kurgyis Z, Keppler S, Pechloff K, Hameister E, Öllinger R, Maresch R, Buch T, Steiger K, Winter C, Rad R, Ruland J
Nature 2017 Dec 7;552(7683):121-125

Intracellular Delivery of Anti-pPKCθ (Thr538) via Protein Transduction Domain Mimics for Immunomodulation.

Ozay EI, Gonzalez-Perez G, Torres JA, Vijayaraghavan J, Lawlor R, Sherman HL, Garrigan DT Jr, Burnside AS, Osborne BA, Tew GN, Minter LM
Molecular therapy : the journal of the American Society of Gene Therapy 2016 Dec;24(12):2118-2130

Inhibition of PKC-θ preserves cardiac function and reduces fibrosis in streptozotocin-induced diabetic cardiomyopathy.

Li Z, Abdullah CS, Jin ZQ
British journal of pharmacology 2014 Jun;171(11):2913-24

PKC-θ exists in an oxidized inactive form in naive human T cells.

von Essen MR, Kongsbak M, Levring TB, Hansen AK, Boding L, Lauritsen JP, Woetmann A, Baier G, Ødum N, Bonefeld CM, Geisler C
European journal of immunology 2013 Jun;43(6):1659-66

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-PKC theta pThr538 in Jurkat cell lysate stimulated with 100 ng/mL PMA for 1 hr using a Phospho-PKC theta pThr538 recombinant rabbit monoclonal antibody (Product # 700043) at a dilution of 3 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKC-theta (pT538) was performed by loading 30 µg of HEK-293 (lane1) HEK-293 treated for 20 minutes with 200 nM of PMA (lane2), Jurkat (lane3) and Jurkat treated for 20 minutes with 200 nM of PMA (lane4) cell lysate using Novex®NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. PKC-theta (pT538) was detected at ~80 kDa using PKC-theta (pT538) Rabbit Monoclonal Antibody (Product # 700043) at 1-2 µg/mL in 5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-PKC theta pThr538 in formalin-fixed, paraffin-embedded human breast carcimona tissue using a Phospho-PKC theta pThr538 monoclonal antibody (Product # 700043) at a dilution of 5 µg/mL. Staining was visualized using DAB. Images were taken at a magnification of 20x. Results show cytoplasmic staining in tumor cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Phospho-PKC theta pThr538 in Jurkat cells treated with 100uM PMA for 1hr using a Phospho-PKC theta pThr538 recombinant rabbit monoclonal antibody (Product # 700043) at a dilution of 0.1 µg. Cells were fixed and permeabilized using FIX & PERM (Product # GAS-004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (gray) compared to samples incubated with a non-phosphopeptide (blue). Pre-incubation with the immunogenic peptide decreased the signal (red) whereas incubation with the non-phosphopeptide did not.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of PKC-theta [pT538] was done on Jurkat cells treated with PMA (200nM, 20 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Tritonª X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with ABfinityª PKC-theta [pT538] Recombinant Rabbit Monoclonal Antibody (700043, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor¨ 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Phospho-PKC theta pThr538 in Jurkat cells treated with 100uM PMA for 1hr using a Phospho-PKC theta pThr538 recombinant rabbit monoclonal antibody (Product # 700043) at a dilution of 0.1 µg. Cells were fixed and permeabilized using FIX & PERM (Product # GAS-004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (gray) compared to samples incubated with a non-phosphopeptide (blue). Pre-incubation with the immunogenic peptide decreased the signal (red) whereas incubation with the non-phosphopeptide did not.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

700043

Antibody data