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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-29029 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PKC theta (Ser695) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, HepG2, Molt-4, Raji, MCF7 (500nM PMA treatment for 1hr).
- Concentration
- 0.66 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Phospho-PKC theta (Ser695) was performed by separating 30 µg of un-treated (-) and treated (+, 500nM PMA treatment for 1 hr) MCF-7 whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Phospho-PKC theta (Ser695) Polyclonal Antibody (Product # PA5-29029) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Phospho-PKC theta (Ser695) Polyclonal Antibody (Product # PA5-29029). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: Hep G2. Lane C: Molt-4 . 7.5% SDS PAGE. PKC theta-phospho-S695 antibody. Phospho-PKC theta (Ser695) Polyclonal Antibody (Product # PA5-29029) diluted at 1:1,000.
