Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NSB970 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NSB970, RRID:AB_10003222
- Product name
- Rabbit Polyclonal PKC theta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Three-dimensional structure of bacteriophage T4 baseplate
Victor A Kostyuchenko, Petr G Leiman, Paul R Chipman, Shuji Kanamaru, Mark J van Raaij, Fumio Arisaka, Vadim V Mesyanzhinov, Michael G Rossmann
Nature Structural Biology 2003 Aug;10(9):688-693
Nature Structural Biology 2003 Aug;10(9):688-693
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKC theta [p Ser676] Antibody [NSB970] - Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on 10% polyacrylamide gel and transferred to PVDF. Membranes were left untreated (1-4) or treated with lambda (l) phosphatase (5), blocked with 5% BSA-TBST buffer ON at 4C, and incubated with 0.50mg/mL PKCq [pS676] Ab for 2hrs at RT in 3% BSA-TBST buffer, following prior incubation with: generic phosphoserine containing peptide (1), non-phosphopeptide corresponding to immunogen (2), phosphopeptide immunogen (3), or no peptide (4, 5). Data show that the peptide corresponding to PKCq [pS676] blocks the Ab signal, thereby demonstrating the specificity of the Ab; and that phosphatase stripping eliminates the signal, verifying that the Ab is phospho-specific.
