Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP02534PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP02534PU-S, RRID:AB_1621152
- Product name
- anti PRKCQ / PRKCT pSer676
- Antibody type
- Polyclonal
- Antigen
- The antiserum was produced against synthesized phosphopeptide derived from human PKCθ around the phosphorylation site of serine 676 (R-L-SP-F-A).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Western blot analysis of extract from Jurkat cells untreated or treated with PMA (1ng/ml, 5min), using PKC-theta antibody AP02782PU (Lane 1 and 2) and PKC-theta pSer676 antibody AP02534PU (Lane 3 and 4).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 4. Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKC-Theta antibody (Phospho-Ser676) AP02534PU-N/AP02534PU-S
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using PKC-theta pSer676 antibody AP02534PU (Red)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKC-theta antibody AP02534PU