AM20219PU-N

antibody from Acris Antibodies GmbH

Targeting: PRKCQ

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20219PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20219PU-N, RRID:AB_10756925

Product name

anti PRKCQ / PRKCT (C-term) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Peptide conjugated to hemocyanin derived from the C-terminal domain of PKC theta.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1C2

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous PKC theta: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDFmembranes. Immunoblots were probed with PKCtheta antibody (Clone 1C2) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: A431 Lane 2:SW480 Lane 3: SW620Lane 4: HT29 Lane 5: MCF-7Lane 6: MDA-MB-231Lane 7: T47D