359301

antibody from BioLegend

Targeting: ITGA2 CD49B

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

359301

Provider

BioLegend

Proper citation

BioLegend Cat#359301, RRID:AB_2562681

Product name

Purified anti-human CD49b

Antibody type

Monoclonal

Antigen

HT1080 cells

Description

CD49b is a 170 kD transmembrane protein, also known as α₂ integrin, VLA-2 α chain, Integrin α₂ and GPIa. It associates with CD29 (β₁ integrin) to form VLA-2, a collagen and laminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

P1E6-C5

Vial size

50 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood platelets stained with purified CD49b (clone P1E6-C5) or purified mouse IgG1, κ isotype control, followed by anti-mouse IgG PE.

Conjugate

Unconjugated