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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MA1-26714 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITGA2 Monoclonal Antibody (31H4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA1-26714 detects Integrin alpha 2 from human samples.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 31H4
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The fourth blade within the beta-propeller is involved specifically in C3bi recognition by integrin alpha M beta 2.
Li Y, Zhang L
The Journal of biological chemistry 2003 Sep 5;278(36):34395-402
The Journal of biological chemistry 2003 Sep 5;278(36):34395-402
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- ITGA2 Monoclonal Antibody (31H4) (Product # MA1-26714) and ~ 160 kDa band corresponding to ITGA2 was observed across the cell lines tested. Membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), T-47D (Lane 2) and U-937 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
