AF1740

antibody from Novus Biologicals

Targeting: ITGA2 CD49B

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1740

Provider

Novus Biologicals

Product name

Sheep Polyclonal Integrin alpha 2/CD49b Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Integrin alpha 2/CD49b in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human Integrin alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 5 and rmIntegrin alpha E is observed.

Reactivity

Mouse

Host

Sheep

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 PE-conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Sheep Anti-Mouse Integrin alpha 2/CD49b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1740) or (B) Normal Sheep IgG Control (Catalog # 5-001-A) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).