GTX63576

antibody from GeneTex

Targeting: ITGA2 CD49B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX63576

Provider

GeneTex

Product name

Integrin alpha 2 antibody [EPR5788]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rabbit

Storage

Store at -20°C. Stable for 12 months at -20°C

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis on (A) T47-D, (B) 293T, (C) human platelet, and (D) A431 lysates using anti-CD49b/Integrin alpha-2 RabMAb (cat. #GTX63576).

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of (1) T47D, (2) 293T, (3) human platelet and (4) A431 lysates (10 ?g per lane) using CD49b/Integrin alpha 2 antibody [EPR5788] at a dilution of 1:10,000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of (1) T47-D and (2) HEK293 lysates (20 μg per lane) using Integrin alpha 2 antibody [EPR5788] at a dilution of 1:10000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of mouse spleen lysate (20 μg) using Integrin alpha 2 antibody [EPR5788] at a dilution of 1:10000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of A431 lysate (20 μg) using Integrin alpha 2 antibody [EPR5788] at a dilution of 1:50000.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Overlay histogram showing A431 cells stained with CD49b/Integrin alpha 2 antibody [EPR5788] (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (CD49b/Integrin alpha 2 antibody [EPR5788], 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa FluorR 488 goat anti-rabbit IgG (H&L) (ab150077) at a dilution of 1:2,000. for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Validation comment

FACS