Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-0500-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD49b (Integrin alpha 2) Monoclonal Antibody (P1H5), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This P1H5 monoclonal antibody reacts with human CD49b, which is also known as integrin alpha 2. Integrins constitute a family of cell surface receptors that mediate adhesion between cells or between a cell and the extracellular matrix. These proteins are heterodimers consisting of two distinct subunits, alpha and beta. Integrins are expressed on hematopoietic, non-hematopoietic, and cancer cells. Integrin-mediated adhesion is regulated by changes in ligand affinity (or activation), cell shape, and integrin clustering and diffusion. Integrin alpha 2 forms a complex with the common beta 1 subunit to form the alpha2beta1 integrin, which binds type I collagen and laminin. Moreover, studies suggest that this complex suppresses metastasis in cancer and participates in T cell costimulation by activating the MAPK and PI3K pathways. The P1H5 monoclonal antibody has been reported to inhibit adhesion of fibroblasts and platelets to collagen. Crossblocking studies indicate that P1H5 and eBioY418 recognize different epitopes. Applications Reported: This P1H5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P1H5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- P1H5
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The α₂β₁ integrin is a metastasis suppressor in mouse models and human cancer.
Alpha2beta1 integrin is the major collagen-binding integrin expressed on human Th17 cells.
Extracellular matrix receptors, ECMRII and ECMRI, for collagen and fibronectin correspond to VLA-2 and VLA-3 in the VLA family of heterodimers.
The human fibroblast class II extracellular matrix receptor mediates platelet adhesion to collagen and is identical to the platelet glycoprotein Ia-IIa complex.
Ramirez NE, Zhang Z, Madamanchi A, Boyd KL, O'Rear LD, Nashabi A, Li Z, Dupont WD, Zijlstra A, Zutter MM
The Journal of clinical investigation 2011 Jan;121(1):226-37
The Journal of clinical investigation 2011 Jan;121(1):226-37
Alpha2beta1 integrin is the major collagen-binding integrin expressed on human Th17 cells.
Boisvert M, Chetoui N, Gendron S, Aoudjit F
European journal of immunology 2010 Oct;40(10):2710-9
European journal of immunology 2010 Oct;40(10):2710-9
Extracellular matrix receptors, ECMRII and ECMRI, for collagen and fibronectin correspond to VLA-2 and VLA-3 in the VLA family of heterodimers.
Takada Y, Wayner EA, Carter WG, Hemler ME
Journal of cellular biochemistry 1988 Aug;37(4):385-93
Journal of cellular biochemistry 1988 Aug;37(4):385-93
The human fibroblast class II extracellular matrix receptor mediates platelet adhesion to collagen and is identical to the platelet glycoprotein Ia-IIa complex.
Kunicki TJ, Nugent DJ, Staats SJ, Orchekowski RP, Wayner EA, Carter WG
The Journal of biological chemistry 1988 Apr 5;263(10):4516-9
The Journal of biological chemistry 1988 Apr 5;263(10):4516-9
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0039-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human CD49b (Integrin alpha 2) APC (right). Cells in the lymphocyte gate were used for analysis.