Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD49b (Integrin alpha 2) Recombinant Rabbit Monoclonal Antibody (SN0752)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SN0752
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD49b (Integrin alpha 2) in A431 cell lysate using a CD49b (Integrin alpha 2) Monoclonal antibody (Product # MA5-32306) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CD49b (Integrin alpha 2) Recombinant Rabbit Monoclonal Antibody (SN0752) (Product # MA5-32306) and bands at 160, 120 kDa corresponding to CD49b were observed across the cell lines and tissue tested except U-937 which is reported to be negative. Membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), T-47D (Lane 3), A-431 (Lane 4), U-2 OS (Lane 5), HEK-293 (Lane 6), A549 (Lane 7), U-937 (Lane 8) and Mouse Spleen (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD49b (Integrin alpha 2) in A431 cells using a CD49b (Integrin alpha 2) Monoclonal antibody (Product # MA5-32306) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD49b (Integrin alpha 2) in MCF-7 cells using a CD49b (Integrin alpha 2) Monoclonal antibody (Product # MA5-32306) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD49b (Integrin alpha 2) of paraffin-embedded Human colon cancer tissue using a CD49b-Integrin-alpha-2 Monoclonal antibody (Product #MA5-32306). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD49b (Integrin alpha 2) of paraffin-embedded Human breast carcinoma tissue using a CD49b-Integrin-alpha-2 Monoclonal antibody (Product #MA5-32306). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD49b (Integrin alpha 2) of paraffin-embedded Human kidney tissue using a CD49b-Integrin-alpha-2 Monoclonal antibody (Product #MA5-32306). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD49b (Integrin alpha 2) of paraffin-embedded Mouse colon tissue using a CD49b-Integrin-alpha-2 Monoclonal antibody (Product #MA5-32306). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD49b (Integrin alpha 2) of paraffin-embedded Mouse stomach tissue using a CD49b-Integrin-alpha-2 Monoclonal antibody (Product #MA5-32306). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of CD49b (Integrin alpha 2) in A431 cells using a CD49b (Integrin alpha 2) Monoclonal Antibody (Product # MA5-32306) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.