Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB1233 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin alpha 2/CD49b Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Integrin alpha 2/CD49b.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- HAS3
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Glycosylation modulates melanoma cell α2β1 and α3β1 integrin interactions with type IV collagen.
Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit.
Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit.
Immunohistochemical demonstration of cellular antigens of the cat defined by anti-human antibodies.
Immunohistochemical demonstration of cellular antigens of the cat defined by anti-human antibodies.
In vitro cultured stromal cells from human tonsils display a distinct phenotype and induce B cell adhesion and proliferation.
Direct binding of collagen to the I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29) in a divalent cation-independent manner.
Cell surface expression and functional significance of adhesion molecules on human myeloma-derived cell lines.
Cell surface expression and functional significance of adhesion molecules on human myeloma-derived cell lines.
Stawikowski MJ, Aukszi B, Stawikowska R, Cudic M, Fields GB
The Journal of biological chemistry 2014 Aug 1;289(31):21591-604
The Journal of biological chemistry 2014 Aug 1;289(31):21591-604
Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit.
Londrigan SL, Graham KL, Takada Y, Halasz P, Coulson BS
Journal of virology 2003 Sep;77(17):9486-501
Journal of virology 2003 Sep;77(17):9486-501
Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit.
Londrigan SL, Graham KL, Takada Y, Halasz P, Coulson BS
Journal of virology 2003 Sep;77(17):9486-501
Journal of virology 2003 Sep;77(17):9486-501
Immunohistochemical demonstration of cellular antigens of the cat defined by anti-human antibodies.
Joling P, Broekhuizen R, de Weger RA, Rottier PJ, Egberink H
Veterinary immunology and immunopathology 1996 Sep;53(1-2):115-27
Veterinary immunology and immunopathology 1996 Sep;53(1-2):115-27
Immunohistochemical demonstration of cellular antigens of the cat defined by anti-human antibodies.
Joling P, Broekhuizen R, de Weger RA, Rottier PJ, Egberink H
Veterinary immunology and immunopathology 1996 Sep;53(1-2):115-27
Veterinary immunology and immunopathology 1996 Sep;53(1-2):115-27
In vitro cultured stromal cells from human tonsils display a distinct phenotype and induce B cell adhesion and proliferation.
Lisignoli G, Monaco MC, Facchini A, Toneguzzi S, Cattini L, Hilbert DM, Lavaroni S, Belvedere O, Degrassi A
European journal of immunology 1996 Jan;26(1):17-27
European journal of immunology 1996 Jan;26(1):17-27
Direct binding of collagen to the I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29) in a divalent cation-independent manner.
Kamata T, Takada Y
The Journal of biological chemistry 1994 Oct 21;269(42):26006-10
The Journal of biological chemistry 1994 Oct 21;269(42):26006-10
Cell surface expression and functional significance of adhesion molecules on human myeloma-derived cell lines.
Kim I, Uchiyama H, Chauhan D, Anderson KC
British journal of haematology 1994 Jul;87(3):483-93
British journal of haematology 1994 Jul;87(3):483-93
Cell surface expression and functional significance of adhesion molecules on human myeloma-derived cell lines.
Kim I, Uchiyama H, Chauhan D, Anderson KC
British journal of haematology 1994 Jul;87(3):483-93
British journal of haematology 1994 Jul;87(3):483-93
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin alpha 2/CD49b in HT1080 Human Cell Line. Integrin alpha 2/CD49b was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Integrin alpha 2/CD49b in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.