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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB12332 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin alpha 2/CD49b Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Integrin alpha 2/CD49b.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P17301- Isotype
- IgG
- Antibody clone number
- 430903
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin alpha 2/CD49b in MG-63 Human Cell Line. Integrin alpha 2/ CD49b was detected in immersion fixed MG-63 human osteosarcoma cell line using Rat Anti-Human Integrin alpha 2/ CD49b Monoclonal Anti-body (Catalog # MAB12332) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Integrin alpha 2/ CD49b in MG63 Human Cell Line by Flow Cytometry. MG63 human osteosarcoma cell line was stained with Rat Anti-Human Integrin alpha 2/CD49b Monoclonal Antibody (Catalog # MAB12332, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
