14173-1-AP

antibody from Proteintech Group

Targeting: CHD4 Mi-2b, Mi2-BETA

Provider product page for 14173-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [15]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 14173-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14173-1-AP, RRID:AB_2080046
Product name: CHD4 antibody
Antibody type: Polyclonal
Description: KD/KO validated CHD4 antibody (Cat. #14173-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CHD4 protein structure - 14173-1-AP shown in red.

Submitted references HDAC11 interacts with the NuRD (MTA3) complex to transcriptionally suppress TGFβ1 expression and inhibit hepatocellular carcinoma metastasis.

Yang Y, Wang J, Wu Q, Wang Y, Meng H, Zeng L, Qiu T, Zhao H, Hu Q, Weng Q, Liu M, Chen M, Qiu R, Ji J, Chen W
Clinical epigenetics 2026 Jan 17;18(1):29

CHD4 drives gastric cancer metastasis via MYH9/GSK3β/β-catenin axis and WNT/EMT pathway activation.

Shi Y, Zhao Z, Zhou S, Zhou Z, Huang Z, Zhou Z, Zhang C
Cancer letters 2025 Sep 28;628:217813

PROX1 is an early driver of lineage plasticity in prostate cancer.

Duan Z, Shi M, Kumaraswamy A, Lin D, Khokhani D, Wang Y, Zhang C, Flores D, Rodansky E, Swaim OA, Storck WK, Beck HN, Patel RA, Sayar E, Hanratty BP, Xue H, Dong X, Maylin ZR, Wan R, Quigley DA, Sjöström M, Hu YM, Zhao F, Xia Z, Cheng S, Yu X, Feng FY, Zhang L, Aggarwal R, Small EJ, Ravikumar V, Rao A, Bedi K, Lee JK, Morrissey C, Coleman I, Nelson PS, Corey E, Udager AM, Rebernick RJ, Cieslik MP, Chinnaiyan AM, Yates JA, Haffner MC, Wang Y, Alumkal JJ
The Journal of clinical investigation 2025 Jun 2;135(11)

O-GlcNAcylated RALY Contributes to Hepatocellular Carcinoma Cells Proliferation by Regulating USP22 mRNA Nuclear Export.

Liu S, Lv Q, Mao X, Dong H, Xu W, Du X, Jia W, Feng K, Zhang J, Zhang Y
International journal of biological sciences 2024;20(9):3675-3690

CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development.

Chen X, Huang MF, Fan DM, He YH, Zhang WJ, Ding JC, Peng BL, Pan X, Liu Y, Du J, Li Y, Liu ZY, Xie BL, Kuang ZJ, Yi J, Liu W
Nucleic acids research 2024 Jul 8;52(12):6811-6829

YAP/TAZ enhances P-body formation to promote tumorigenesis.

Shen X, Peng X, Guo Y, Dai Z, Cui L, Yu W, Liu Y, Liu CY
eLife 2024 Jul 24;12

Circular RNA circWBSCR22 facilitates colorectal cancer metastasis by enhancing CHD4's protein stability.

Luo J, Xu S, Wang J, He L, Li Z
International journal of biological macromolecules 2024 Dec;282(Pt 4):137135

Post-translational control of beige fat biogenesis by PRDM16 stabilization.

Wang Q, Li H, Tajima K, Verkerke ARP, Taxin ZH, Hou Z, Cole JB, Li F, Wong J, Abe I, Pradhan RN, Yamamuro T, Yoneshiro T, Hirschhorn JN, Kajimura S
Nature 2022 Sep;609(7925):151-158

Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAF.

Sun X, Cheng L, Sun Y
Molecular autism 2022 Jun 1;13(1):24

CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer.

Zhang J, Lv X, Wei B, Gong X, Chen L
Cellular signalling 2022 Dec;100:110464

Functional Genomic Analysis of a RUNX3 Polymorphism Associated With Ankylosing Spondylitis.

Vecellio M, Chen L, Cohen CJ, Cortes A, Li Y, Bonham S, Selmi C, Brown MA, Fischer R, Knight JC, Wordsworth BP
Arthritis & rheumatology (Hoboken, N.J.) 2021 Jun;73(6):980-990

ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.

Lan X, Ren R, Feng R, Ly LC, Lan Y, Zhang Z, Aboreden N, Qin K, Horton JR, Grevet JD, Mayuranathan T, Abdulmalik O, Keller CA, Giardine B, Hardison RC, Crossley M, Weiss MJ, Cheng X, Shi J, Blobel GA
Molecular cell 2021 Jan 21;81(2):239-254.e8

CHD4 Promotes Breast Cancer Progression as a Coactivator of Hypoxia-Inducible Factors.

Wang Y, Chen Y, Bao L, Zhang B, Wang JE, Kumar A, Xing C, Wang Y, Luo W
Cancer research 2020 Sep 15;80(18):3880-3891

CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.

Shao S, Cao H, Wang Z, Zhou D, Wu C, Wang S, Xia D, Zhang D
Clinical epigenetics 2020 Feb 18;12(1):31

Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development.

Vishwakarma BA, Nguyen N, Makishima H, Hosono N, Gudmundsson KO, Negi V, Oakley K, Han Y, Przychodzen B, Maciejewski JP, Du Y
Leukemia 2016 Jan;30(1):200-8

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 14173-1-AP(CHD4 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of A549 cells, using CHD4 antibody 14173-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human cervical cancer using 14173-1-AP(CHD4 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue