Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-32181 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHD4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (100%), Xenopus laevis (82%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CHD4 Polyclonal Antibody (Product # PA5-32181). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2. 5% SDS PAGE. CHD4 Polyclonal Antibody (Product # PA5-32181) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CHD4 in paraformaldehyde-fixed HeLa cells using a CHD4 polyclonal antibody (Product # PA5-32181) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHD4 Polyclonal Antibody detects CHD4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. CHD4 Polyclonal Antibody (Product # PA5-32181) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHD4 Polyclonal Antibody detects CHD4 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. CHD4 Polyclonal Antibody (Product # PA5-32181) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded D54 xenograft, using CHD4 (Product # PA5-32181) antibody at 1:1,000 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.