Explore
Validate
Learn
Immunocytochemistry
Immunohistochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- HPA012008 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA012008, RRID:AB_1846633
- Product name
- Anti-CHD4
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human CHD4, Gene description: chromodomain helicase DNA binding protein 4, Alternative Gene Names: Mi-2b, Mi2-BETA, Validated applications: IHC, ICC, ChIP, Uniprot ID: Q14839, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references RNAi screens identify CHD4 as an essential gene in breast cancer growth
D'Alesio C, Punzi S, Cicalese A, Fornasari L, Furia L, Riva L, Carugo A, Curigliano G, Criscitiello C, Pruneri G, Pelicci P, Faretta M, Bossi D, Lanfrancone L
Oncotarget 2016;7(49):80901-80915
Oncotarget 2016;7(49):80901-80915
No comments: Submit comment
Enhanced validation
- Submitted by
- 55af80e3e0991
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal images of immunofluorescently stained human U-2 OS cells.The protein CHD4 is shown in green. The image to the left show cells transfected with control siRNA and the image to the right show cells where CHD4 has been downregulated with specific siRNA.
- Sample type
- U-2 OS cells
- Primary Ab dilution
- 1:24
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:800
- Knockdown/Genetic Approaches Application
- Immunocytochemistry
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-CHD4 (HPA012008, Lot 000018062) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
