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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PAB3193 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3193, RRID:AB_1579170
- Product name
- PRKG2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKG2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Autophosphorylation of cGMP-dependent protein kinase type II.
Inhibition of cGMP-dependent protein kinase II by its own splice isoform.
Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase.
Vaandrager AB, Hogema BM, Edixhoven M, van den Burg CM, Bot AG, Klatt P, Ruth P, Hofmann F, Van Damme J, Vandekerckhove J, de Jonge HR
The Journal of biological chemistry 2003 Aug 1;278(31):28651-8
The Journal of biological chemistry 2003 Aug 1;278(31):28651-8
Inhibition of cGMP-dependent protein kinase II by its own splice isoform.
Gambaryan S, Palmetshofer A, Glazova M, Smolenski A, Kristjansson GI, Zimmer M, Lohmann SM
Biochemical and biophysical research communications 2002 May 24;293(5):1438-44
Biochemical and biophysical research communications 2002 May 24;293(5):1438-44
Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase.
Orstavik S, Solberg R, Taskén K, Nordahl M, Altherr MR, Hansson V, Jahnsen T, Sandberg M
Biochemical and biophysical research communications 1996 Mar 27;220(3):759-65
Biochemical and biophysical research communications 1996 Mar 27;220(3):759-65
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKG2 (arrow) using PRKG2 polyclonal antibody (Cat # PAB3193).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKG2 gene (Lane 2).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MC3T3-E1 cell lysate with PRKG2 polyclonal antibody (Cat # PAB3193).Lane 1, membrane extracts.Lane 2, cytosol extracts.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKG2 polyclonal antibody (Cat # PAB3193) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinomareacted with PRKG2 polyclonal antibody (Cat # PAB3193), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
