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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- MA1-843 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCoR2 Monoclonal Antibody (1542)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA1-843 detects silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) from human cells and tissues.
- Antibody clone number
- 1542
- Concentration
- 1 mg/mL
Submitted references Regulation of subnuclear localization is associated with a mechanism for nuclear receptor corepression by RIP140.
Tazawa H, Osman W, Shoji Y, Treuter E, Gustafsson JA, Zilliacus J
Molecular and cellular biology 2003 Jun;23(12):4187-98
Molecular and cellular biology 2003 Jun;23(12):4187-98
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of NCoR2 was performed by loading 25 µg of Hela (lane 1) and Neuro-2a (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a NCoR2 monoclonal antibody (Product # MA1-843) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~220-250 kDa in Hela cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
