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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-23991 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRSS3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Host neuronal PRSS3 interacts with enterovirus A71 3A protein and its role in viral replication.
In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer.
Rattanakomol P, Srimanote P, Tongtawe P, Khantisitthiporn O, Supasorn O, Thanongsaksrikul J
Scientific reports 2022 Jul 27;12(1):12846
Scientific reports 2022 Jul 27;12(1):12846
In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer.
Aboulouard S, Wisztorski M, Duhamel M, Saudemont P, Cardon T, Narducci F, Lemaire AS, Kobeissy F, Leblanc E, Fournier I, Salzet M
Cell reports. Medicine 2021 Jun 15;2(6):100318
Cell reports. Medicine 2021 Jun 15;2(6):100318
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRSS3 in formalin fixed and paraffin embedded human pancreas tissue. Samples were incubated with PRSS3 polyclonal antibody (Product # PA5-23991) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MDA-MB231 cells using a PRSS3 polyclonal antibody (Product # PA5-23991) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 ( a ) Agarose gel electrophoresis of PRSS1, PRSS2, PRSS3 , and Pan-PRSS amplicons determined in HEK293T and SH-SY5Y cells by RT-PCR using specific primers. The sizes of the PCR amplicons of PRSS1 , PRSS2 , PRSS3, and Pan-PRSS are 177, 180, 183, and 265 bp, respectively. ( b ) Micrographs of fluorescent signals from HEK293T and SH-SY5Ycells stained with anti-PRSS3 polyclonal antibody to detect endogenous PRSS3 protein expression. ( c ) Agarose gel electrophoresis of amplicons of full-length PRSS3 transcript variants determined in SH-SY5Y cells by RT-PCR with gradient annealing temperatures. Individual full-length transcript variant of PRSS3 were amplified by RT-PCR using annealing temperatures at 50, 55, and 60 degC. Only PRSS3 transcript variant 3 ( PRSS3-V3 ) at the size of 798 bp could be amplified using annealing temperature at 60 degC as indicated by the arrows. Lane M, 100 bp Plus DNA ladder (in base pairs, bp). Lane NTC or N, non-template control.
