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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP53470PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53470PU-N, RRID:AB_11147442
- Product name
- anti Trypsin-3 / PRSS3 (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 20-48aa) of human Trypsin-3 / PRSS3
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in MDA-MB231 cell line lysates (35ug/lane) using Trypsin-3 / PRSS3 antibody Cat.-No. AP53470PU-N (N-term). This demonstrates the PRSS3 antibody detected the PRSS3 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human pancreas tissue (Formalin-fixed, Paraffin-embedded) using Trypsin-3 / PRSS3 antibody Cat.-No. AP53470PU-N (N-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRSS3 antibody for IHC; Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram) using Trypsin-3 / PRSS3 antibody Cat.-No. AP53470PU-N (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
