IC26051P

antibody from Novus Biologicals

Targeting: GATA3 HDR

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC26051P

Provider

Novus Biologicals

Product name

Mouse Monoclonal GATA-3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human GATA-1, -2, -4, -5, or -6.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis.

Costa SF, Gomes VO, Dos Santos Maciel MO, Melo LM, Venturin GL, Bragato JP, Rebech GT, de Oliveira Santos C, Nascimento de Oliveira BM, Gileno de Sá Oliveira G, Felix de Lima VM
PLoS neglected tropical diseases 2020 Jan;14(1):e0008021

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Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes.

Melo LM, Bragato JP, Venturin GL, Rebech GT, Costa SF, Garcia LE, Lopes FL, Eugênio FR, Patto Dos Santos PS, de Lima VMF
PloS one 2019;14(12):e0226192

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of GATA-3 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human GATA-3 PE-conjugated Monoclonal Antibody (Catalog # IC26051P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.