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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA809143 - Provider product page
- Provider
- OriGene
- Product name
- GATA3 mouse monoclonal antibody, clone OTI4D5 (formerly 4D5)
- Antibody type
- Monoclonal
- Description
- GATA3 mouse monoclonal antibody, clone OTI4D5 (formerly 4D5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GATA3
- Isotype
- IgG
- Antibody clone number
- OTI4D5
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MDA-MB-468 cells using anti-GATA3 mouse monoclonal antibody (TA809143)(Left). The right is negative control.(1:10000)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MCF-7 cells using anti-GATA3 mouse monoclonal antibody (TA809143)(Left). The right is negative control.(1:10000)
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of penetrated MCF-7 cells, using anti-GATA3 antibody(TA809143),(Red), compared to a nonspecific negative control antibody,(Blue). The right is HUVEC cells as negtive control.(1:100)
- Validation comment
- FC
