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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN2508189 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-GATA Binding Protein 3 (GATA3) (Center) antibody
- Antibody type
- Monoclonal
- Antigen
- This GATA3 antibody is generated from a mouse immunized with a recombinant protein from human GATA3.Antigen type: Recombinant Protein
- Description
- This antibody is purified through a protein G column, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Mouse
- Epitope
- Center
- Isotype
- IgG
- Vial size
- 200 μL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- Western Blot of ATTO 488 conjugated Goat anti-Mouse IgG (Pre-Adsorbed) secondary antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 488 goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): none. ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence
