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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- UM800110 - Provider product page
- Provider
- OriGene
- Product name
- GATA3 mouse monoclonal antibody,clone UMAB218
- Antibody type
- Monoclonal
- Description
- GATA3 mouse monoclonal antibody,clone UMAB218
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GATA3
- Isotype
- IgG
- Antibody clone number
- UMAB218
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MCF-7 cells using anti-GATA3 mouse monoclonal antibody (UM800110)(Left). The right is negative control.((1:2000)
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human urothelial carcinoma using anti-GATA3 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800110 was diluted 1:50 and detection was done with HRP secondary and DAB chromogen. Strong nuclear stain is seen in the tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human kidney tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800110 was diluted 1:50, detection was done with HRP secondary and DAB chromogen. Weak nuclear stain is seen in the tubule epithelial cells of the kidney.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded carcinoma of human prostate tissue using anti-GATA3 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800110 was diluted 1:50 and detection was done with HRP secondary and DAB chromogen. Stong nuclear stain was seen in the tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded at margins of adenocarcinoma of human breast tissue using anti-GATA3 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800110 was diluted 1:50 and detection was done with HRP secondary and DAB chromogen. Strong nuclear stain is seen in the epithelial cells.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-GATA3 mouse monoclonal antibody (UM800110). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
- Validation comment
- 10K-CHIP
