Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB26052-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GATA-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human GATA-3 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human GATA-3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26052) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GATA-3 full length (FL) at approximately 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of GATA-3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th2 cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26052) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.