H00003148-M02

antibody from Abnova Corporation

Targeting: HMGB2 HMG2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003148-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003148-M02, RRID:AB_464063

Product name

HMGB2 monoclonal antibody (M02), clone 4G7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant HMGB2.

Antigen sequence

MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSS
VNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKAR
YDREMKNYVPPKGDKKGKKKDPNAPKRPPSAFFLF
CSEHRPKIKSEHPGLSIGDTAKKLGEMWSEQSAKD
KQPYEQKAAKLKEKYEKDIAAYRAKGKSEAGKKGP
GRPTGSKKKNEPEDEEEEEE


Isotype

IgG

Antibody clone number

4G7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HMGB2 monoclonal antibody (M02), clone 4G7 Western Blot analysis of HMGB2 expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HMGB2 expression in transfected 293T cell line by HMGB2 monoclonal antibody (M02), clone 4G7.Lane 1: HMGB2 transfected lysate(22 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HMGB2 monoclonal antibody (M02), clone 4G7. Western Blot analysis of HMGB2 expression in Hela S3 NE ( Cat # L013V3 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol