H00003148-M05

antibody from Novus Biologicals

Targeting: HMGB2 HMG2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003148-M05

Provider

Novus Biologicals

Proper citation

Novus Cat#H00003148-M05, RRID:AB_921694

Product name

Mouse Monoclonal HMGB2 Antibody

Antibody type

Monoclonal

Description

IgG purified. HMGB2 (3E5)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Genomic interaction between ER and HMGB2 identifies DDX18 as a novel driver of endocrine resistance in breast cancer cells.

Redmond AM, Byrne C, Bane FT, Brown GD, Tibbitts P, O'Brien K, Hill AD, Carroll JS, Young LS
Oncogene 2015 Jul;34(29):3871-80

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: HMGB2 Antibody (3E5) [H00003148-M05] - Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of monoclonal antibody to HMGB2 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml