Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-62773 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AKGKSEAGKK GPGRPTGSKK KNEP Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 96%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of genes functionally involved in the detrimental effects of mutant histone H3.3-K27M in Drosophila melanogaster.
Berlandi J, Chaouch A, De Jay N, Tegeder I, Thiel K, Shirinian M, Kleinman CL, Jeibmann A, Lasko P, Jabado N, Hasselblatt M
Neuro-oncology 2019 May 6;21(5):628-639
Neuro-oncology 2019 May 6;21(5):628-639
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG. Samples were probed using a HMGB2 Polyclonal Antibody (Product # PA5-62773).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB2 in human cell lines U2OS and A-549 using a HMGB2 Polyclonal Antibody (Product # PA5-62773). Corresponding HMGB2 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HMGB2 in human cell line U-2 OS shows positivity in nucleus & nucleoli. Samples were probed using a HMGB2 Polyclonal Antibody (Product # PA5-62773).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HMGB2 in human bone marrow using HMGB2 Polyclonal Antibody (Product # PA5-62773) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HMGB2 in human cerebral cortex using HMGB2 Polyclonal Antibody (Product # PA5-62773) shows low expression as expected.