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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-78005 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGB1/HMGB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2, A375, mouse brain, rat brain
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1/HMGB2 in non-transfected (-) and transfected (+) 293T cells using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with HMGB1/HMGB2 polyclonal antibody (Product # PA5-78005) using a dilution of 1:20,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1/HMGB2 in rat tissue extract using 50 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with HMGB1/HMGB2 polyclonal antibody (Product # PA5-78005) using a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 Polyclonal Antibody detects HMGB2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract. E. 30 µg A375 whole cell lysate/extract.12 % SDS-PAGE. HMGB1/HMGB2 Polyclonal Antibody (Product # PA5-78005) dilution: 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HMGB1/HMGB2 Polyclonal Antibody (Product # PA5-78005). Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 Polyclonal Antibody (Product # PA5-78005) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HMGB1/HMGB2 Polyclonal Antibody (Product # PA5-78005). Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 Polyclonal Antibody (Product # PA5-78005) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 Polyclonal Antibody detects HMGB2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract. E. 30 µg A375 whole cell lysate/extract.12 % SDS-PAGE. HMGB1/HMGB2 Polyclonal Antibody (Product # PA5-78005) dilution: 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB1/HMGB2 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1/HMGB2 protein stained by HMGB1/HMGB2 antibody (Product # PA5-78005) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB1/HMGB2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1/HMGB2 protein stained by HMGB1/HMGB2 antibody (Product # PA5-78005) diluted at 1:2,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB2 protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HMGB1/HMGB2 antibody (Product # PA5-78005) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB2 protein at nucleus on human duodenal adenocarcinoma tissue by immunohistochemical analysis. Sample: Paraffin-embedded human duodenal adenocarcinoma tissue. HMGB1/HMGB2 antibody (Product # PA5-78005) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
