MA5-35771

antibody from Invitrogen Antibodies

Targeting: HMGB2 HMG2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35771

Provider

Invitrogen Antibodies

Product name

HMGB2 Recombinant Rabbit Monoclonal Antibody (8P10V5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

8P10V5

Vial size

100 µL

Concentration

0.14 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal imaging (Immunocytochemistry) of HMGB2 in U-2 OS cells. Samples were incubated with HMGB2 Monoclonal antibody (Product # MA5-35771) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of HMGB2 in 300 μg extracts from Mouse testis. Samples were precipitated with 3 μg HMGB2 Monoclonal antibody (Product # MA5-35771). Western blot was performed from the immunoprecipitate using HMGB2 Monoclonal antibody (Product # MA5-35771) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HMGB2 in paraffin-embedded rat testis. Samples were incubated with HMGB2 Monoclonal antibody (Product # MA5-35771) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HMGB2 in paraffin-embedded human colon. Samples were incubated with HMGB2 Monoclonal antibody (Product # MA5-35771) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HMGB2 in paraffin-embedded mouse testis. Samples were incubated with HMGB2 Monoclonal antibody (Product # MA5-35771) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of HMGB2 in extracts from HepG2 cells. Samples were incubated with HMGB2 Monoclonal antibody (Product # MA5-35771) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.