Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- RZ1169 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Zebrafish Hmgb2 Antibody / Hmgb2a / Hmgb2b
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Zebrafish
- Host
- Rabbit
- Vial size
- 100 ug
- Concentration
- Lyophilized
- Storage
- After reconstitution, the Zebrafish Hmgb2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of Hmgb2a/b protein using Zebrafish Hmgb2 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~24 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish brain tissue with Zebrafish Hmgb2 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish esophagus tissue with Zebrafish Hmgb2 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish colon tissue with Zebrafish Hmgb2 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish muscle tissue with Zebrafish Hmgb2 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.