LS-C785400

antibody from LSBio

Targeting: LMAN1 ERGIC-53, ERGIC53, F5F8D, FMFD1, gp58, MCFD1, MR60

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C785400

Provider

LSBio

Product name

ERGIC-53 / LMAN1 Antibody (clone OTI1E3, Carrier-free) LS-C785400

Antibody type

Monoclonal

Description

Purified from ascites.

Reactivity

Human, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1E3

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human thyroid tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human lymph node tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human thyroid tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human lymph node tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Human thyroid tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Human lymph node tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).