Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6359 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6359, RRID:AB_1572111
- Product name
- CARD11 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of CARD11.
- Antigen sequence
C-QRKTIWVDEDQL
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B.
Bertin J, Wang L, Guo Y, Jacobson MD, Poyet JL, Srinivasula SM, Merriam S, DiStefano PS, Alnemri ES
The Journal of biological chemistry 2001 Apr 13;276(15):11877-82
The Journal of biological chemistry 2001 Apr 13;276(15):11877-82
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CARD11 polyclonal antibody (Cat # PAB6359) staining (1 ug/mL) of human Jurkat cells (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CARD11 polyclonal antibody (Cat # PAB6359) (2 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)