Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB13783 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB13783, RRID:AB_10547568
- Product name
- CARD11 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against CARD11.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CARD11 by CARD11 polyclonal antibody (Cat # PAB13783). Jurkat T cells (8 X 106) were activated with CD3 monoclonal antibody (TR66, 10 ug/mL) and protein distribution of lipid raft (R) or non-raft (S) fractions was analyzed by detergent fractionation and immunoblotting (1 : 2,000) with CARD11 in nontransfected Jurkat T cells.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CARD11 by CARD11 polyclonal antibody (Cat # PAB13783). Human T cells (clone 6396p5.1.2) were incubated with CD3 monoclonal antibody. Cells were stained for CD3 (green), phosphotyrosine (red) and CARD11 polyclonal antibody (Cat # PAB13783) (1 : 100 dilution; blue) and analyzed by confocal microscopy.
- Validation comment
- Immunofluorescence