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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A302-541A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARMA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CARMA1 by western blot. Samples: Whole cell lysate from Ramos (5, 15, and 50 µg) and K-562 (K; 50 µg) cells. Antibody: Affinity purified rabbit anti-CARMA1 antibody (Product # A302-541A; lot A302-541A-1) used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CARMA1 by western blot and immunoprecipitation. Samples: Whole cell lysate from Ramos (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and K562 (K; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CARMA1 antibody A302-541A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. CARMA1 was also immunoprecipitated by rabbit anti-CARMA1 antibodies A302-542A, A302-543A and A302-544A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CARMA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Ramos cells. Antibodies: Affinity purified rabbit anti-CARMA1 antibody (Product # A302-541A; lot A302-541A-1) used for IP at 10 µg/mg lysate. CARMA1 was also immunoprecipitated by rabbit anti-CARMA1 antibodies (Product # A302-542A, A302-543A and A302-544A), which recognize downstream epitopes. For blotting immunoprecipitated CARMA1, Product # A302-541A was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 10 seconds.
