Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29702 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARD11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, K562, NCI-H929, BCL-1. Predicted reactivity: Mouse (96%), Chicken (87%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.02 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CARD11 Polyclonal Antibody detects CARD11 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract.5% SDS-PAGE. CARD11 Polyclonal Antibody (Product # PA5-29702) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CARD11 Polyclonal Antibody detects CARD11 protein by western blot analysis. A. 30 µg Raji whole cell lysate/extract. B. 30 µg K562 whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.5% SDS-PAGE. CARD11 Polyclonal Antibody (Product # PA5-29702) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CARD11 (Product # PA5-29702) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
