Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-27169 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCKBR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Unboiled HeLa cell extract.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of CCK-BR using 30 µg of A) A431 (B) H1299 and C) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CCK-BR polyclonal antibody (Product # PA5-27169) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCKBR was performed by separating 30 µg of unboiled HeLa cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CCKBR Polyclonal Antibody (Product # PA5-27169) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CCKBR Polyclonal Antibody detects CCKBR protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: CCKBR Polyclonal Antibody (Product # PA5-27169) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CCKBR Polyclonal Antibody detects CCKBR protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. CCKBR Polyclonal Antibody (Product # PA5-27169) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.