100-401-250

antibody from Rockland Immunochemicals, Inc.

Targeting: BIVM FLJ20159

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-250

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-250, RRID:AB_2064514

Product name

Anti-BIVM (C-terminal specific) (RABBIT) Antibody - 100-401-250

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot of anti-human BIVM. Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody. A 57 kDa band corresponding to human BIVM protein is detected using a 1:1,000 dilution of the antiserum incubated for 1 hour at room temperature. Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) code 611-103-122 diluted 1:10,000 and incubated for 1 hour at room temperature. The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure). Other detection methods will yield similar results.

Validation comment

Western Blot