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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00011164-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00011164-M04, RRID:AB_2154110
- Product name
- Mouse Monoclonal ADP-Sugar Pyrophosphatase/NUDT5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NUDT5 (2A3)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04] - Western blot analysis of NUDT5 over-expressed 293 cell line, cotransfected with NUDT5 Validated Chimera RNAi or non-transfected control. Blot probed with H00011164-M04. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04] - Analysis of NUDT5 expression in transfected 293T cell line by NUDT5 monoclonal antibody (M04), clone 2A3. Lane 1: NUDT5 transfected lysatE (24.3 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (2A3) [H00011164-M04] - Detection limit for recombinant GST tagged NUDT5 is approximately 1ng/ml as a capture antibody.
