PA5-112142

antibody from Invitrogen Antibodies

Targeting: NUDT5 hYSAH1, YSA1H

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112142

Provider

Invitrogen Antibodies

Product name

NUDT5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product can be used in direct ELISA.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NUDT5 using NUDT5 Polyclonal Antibody (Product # PA5-112142) at a dilution of 1:500. Lane A: NUDT5 konckout Hela whole cell lysate, Lane B: Hela whole cell lysate (Lysates/proteins at 10 µMicro;g per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NUDT5 using NUDT5 Polyclonal Antibody (Product # PA5-112142) at a dilution of 1:500. Lane A: Hela whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of NUDT5 was performed on (Lane A) 0.5 mg Hela whole cell lysate using 2 µL of NUDT5 Polyclonal Antibody (Product # PA5-112142) at a dilution of 200, and 15 µL of 50 % Protein G agarose. A Dylight 800-labeled antibody to rabbit IgG (H+L) was used as a secondary antibody at a dilution of 1:5000. Developed using the odssey technique and performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of NUDT5 was performed on (Lane A) 0.5 mg Hela whole cell lysate using 2 µL of NUDT5 Polyclonal Antibody (Product # PA5-112142) at a dilution of 200, and 15 µL of 50 % Protein G agarose. A Dylight 800-labeled antibody to rabbit IgG (H+L) was used as a secondary antibody at a dilution of 1:5000. Developed using the odssey technique and performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa.