RQ8353

antibody from NSJ Bioreagents

Targeting: NUDT5 hYSAH1, YSA1H

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ8353

Provider

NSJ Bioreagents

Product name

NUDT5 Antibody / ADP-sugar pyrophosphatase

Antibody type

Polyclonal

Description

This highly specific NUDT5 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence/Immunoprecipitation/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the NUDT5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human 293T, 3) human HepG2, 4) human Jurkat and 5) rat RH35 cell lysate with NUDT5 antibody. Predicted molecular weight ~35 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human Caco-2, 2) human 293T, 3) human Jurkat, 4) human HepG2 and 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse HEPA1-6 cell lysate with NUDT5 antibody. Predicted molecular weight ~35 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with NUDT5 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.

Immunoprecipitation

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunoprecipitation of NUDT5 protein from 500ug of human 293T whole cell lysate with 2ug of NUDT5 antibody.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer tissue with NUDT5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with NUDT5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with NUDT5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse liver tissue with NUDT5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat liver tissue with NUDT5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human 293T cells with NUDT5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NUDT5 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human Caco-2 cells with NUDT5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NUDT5 antibody.