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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF6414 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human ADP-Sugar Pyrophosphatase/NUDT5 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9UKK9- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human ADP-Sugar Pyrophosphatase/ NUDT5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ADP-Sugar Pyrophosphatase/NUDT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6414) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADP-Sugar Pyrophosphatase/NUDT5 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
