Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LF-MA0155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CDK6 Monoclonal Antibody (75B9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is HepG2 cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 75B9
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of Jurkat (Lane 1), IM-9 (Lane 2), U-87 MG (Lane 3), T98G (Lane 4), A549 (Lane 5), and MCF7 (Lane 6). The blot was probed with Mouse Anti-CDK6 Monoclonal Antibody (Product # LF-MA0155, 1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 38 kDa band corresponding to CDK6 was observed across the cell lines tested. Additional bands observed in the 170 -200 kDa range potentially correspond to the Cyclin D-cdk6 complex as reported in literature. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).