LF-MA0155

antibody from Invitrogen Antibodies

Targeting: CDK6 PLSTIRE

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

LF-MA0155

Provider

Invitrogen Antibodies

Product name

Anti-CDK6 Monoclonal Antibody (75B9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is HepG2 cells.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

75B9

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of Jurkat (Lane 1), IM-9 (Lane 2), U-87 MG (Lane 3), T98G (Lane 4), A549 (Lane 5), and MCF7 (Lane 6). The blot was probed with Mouse Anti-CDK6 Monoclonal Antibody (Product # LF-MA0155, 1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 38 kDa band corresponding to CDK6 was observed across the cell lines tested. Additional bands observed in the 170 -200 kDa range potentially correspond to the Cyclin D-cdk6 complex as reported in literature. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).