Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-27978 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (85%), Chicken (93%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.01 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Human genome-wide RNAi screen reveals host factors required for enterovirus 71 replication.
Wu KX, Phuektes P, Kumar P, Goh GY, Moreau D, Chow VT, Bard F, Chu JJ
Nature communications 2016 Oct 17;7:13150
Nature communications 2016 Oct 17;7:13150
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK6 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CDK6 polyclonal antibody (Product # PA5-27978) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CDK6 Polyclonal Antibody (Product # PA5-27978). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 Polyclonal Antibody (Product # PA5-27978) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CDK6 Polyclonal Antibody (Product # PA5-27978) and a 37 kDa band corresponding to CDK6 was observed in Jurkat, MOLT-4 but not in HeLa and MCF7. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), MOLT-4 (Lane 2), HeLa (Lane 3) and MCF7 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CDK6 in paraformaldehyde-fixed HeLa cells using a CDK6 polyclonal antibody (Product # PA5-27978) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CDK6 was performed using 70% confluent log phase MOLT-4 and MCF7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with CDK6 Polyclonal Antibody (Product # PA5-27978) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents MCF7 having no expression of CDK6. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CDK6 was performed in paraffin-embedded human lung adenocarcinoma tissue using CDK6 Polyclonal Antibody (Product # PA5-27978) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDK6 Polyclonal Antibody detects CDK6 protein at cytoplasm and nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. CDK6 Polyclonal Antibody (Product # PA5-27978) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDK6 Polyclonal Antibody detects CDK6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. CDK6 stained by CDK6 Polyclonal Antibody (Product # PA5-27978) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL