Antibody data
CDK1 protein structure - 200-301-160 shown in red.
Supportive validation
- Submitted by
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Rockland Immunochemicals, Inc. (provider)
- Main image

- Experimental details
- Mab anti-Human p34cdc2 antibody (clone POH-1) is shown to detect human p34cdc2by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p34cdc2at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000. A doublet band corresponding to human p34cdc2is detected at ~34 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p34cdc2. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot