14876-1-AP

antibody from Proteintech Group

Targeting: DAAM1 KIAA0666

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14876-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14876-1-AP, RRID:AB_2089444

Product name

DAAM1 antibody

Antibody type

Polyclonal

Description

KD/KO validated DAAM1 antibody (Cat. #14876-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Polycystin-1 Mutant Alters Mechanotransduction in Response to Collagen and Extracellular Matrix Stiffness via Daam1-Dependent Microfilament Remodeling.

Zhou J, Deng Y, Mei J, Wan M, Xue N, Zong X, Zhou J, Ding J, Yan T, Jiang Y, Xia T, Zheng P, Zhu Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Nov;12(41):e09846

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Mesenchymal stromal/stem cell spheroid-derived extracellular vesicles advance the therapeutic efficacy of 3D-printed vascularized artificial liver lobules in liver failure treatment.

Zhang J, Chen X, Chai Y, Jin Y, Li F, Zhuo C, Xu Y, Wang H, Ju E, Lao YH, Xie X, Li M, Tao Y
Bioactive materials 2025 Jul;49:121-139

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Formin protein DAAM1 positively regulates PD-L1 expression via mediating the JAK1/STAT1 axis in pancreatic cancer.

Xu R, Wan M, Pan J, Mei J, Zhou J, Shen Y, Yang J, Zhu Y, Sun J
Cancer cell international 2025 Jan 29;25(1):28

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Prenatal Perfluorooctanoic Acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4/β-catenin signaling pathway in progeny.

Du H, Song L, Zhao M, Zhao X, Mu R, Gao S, Zhang B, Wang J
Ecotoxicology and environmental safety 2025 Feb;291:117816

---

Hsa_circ_0125356 promotes gemcitabine resistance by modulating WNT canonical and non-canonical pathways via miR-582-5p/FGF9 axis in non-small cell lung cancer.

Du X, Luo W, Li H, Gu Q, Huang P, Wang C, Li N, Liu F, Xia C
Molecular cancer 2025 Feb 27;24(1):59

---

The membrane skeleton is constitutively remodeled in neurons by calcium signaling.

Heller E, Kurup N, Zhuang X
Science (New York, N.Y.) 2025 Aug 7;389(6760):eadn6712

---

C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness.

Ma J, Yang X, Li Y, Zhang X, Liu K, Peng Y, Wang S, Shi R, Huo X, Liu X, Li X, Ye R, Zhang Z, Yang C, Liu L, Gao D, Jia S, Sun L, Zuo X, Meng Q, Chen X
Signal transduction and targeted therapy 2025 Apr 2;10(1):105

---

Integrated proteogenomic characterization of glioblastoma evolution.

Kim KH, Migliozzi S, Koo H, Hong JH, Park SM, Kim S, Kwon HJ, Ha S, Garofano L, Oh YT, D'Angelo F, Kim CI, Kim S, Lee JY, Kim J, Hong J, Jang EH, Mathon B, Di Stefano AL, Bielle F, Laurenge A, Nesvizhskii AI, Hur EM, Yin J, Shi B, Kim Y, Moon KS, Kwon JT, Lee SH, Lee SH, Gwak HS, Lasorella A, Yoo H, Sanson M, Sa JK, Park CK, Nam DH, Iavarone A, Park JB
Cancer cell 2024 Mar 11;42(3):358-377.e8

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Estrogen influences the transzonal projection assembly of cumulus-oocyte complexes through G protein-coupled estrogen receptor during goat follicle development.

Xu R, Wen D, Yin L, Tang Y, Lu S, Gao Y, Pan MH, Han B, Ma B
Molecular reproduction and development 2024 Jun;91(6):e23763

---

Chiral growth of adherent filopodia.

Li W, Chung WL, Kozlov MM, Medalia O, Geiger B, Bershadsky AD
Biophysical journal 2023 Sep 19;122(18):3704-3721

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Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Mei J, Cai Y, Wang H, Xu R, Zhou J, Lu J, Yang X, Pan J, Liu C, Xu J, Zhu Y
Clinical immunology (Orlando, Fla.) 2023 Jan;246:109204

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Characterization of Expression and Function of the Formins FHOD1, INF2, and DAAM1 in HER2-Positive Breast Cancer.

Peippo M, Gardberg M, Kronqvist P, Carpén O, Heuser VD
Journal of breast cancer 2023 Dec;26(6):525-543

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The formin DAAM1 regulates the deubiquitinase activity of USP10 and integrin homeostasis.

Phillips AT, Boumil EF, Venkatesan A, Tilstra-Smith C, Castro N, Knox BE, Henty-Ridilla JL, Bernstein AM
European journal of cell biology 2023 Dec;102(4):151347

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Aberrant centrosome biogenesis disrupts nephron and collecting duct progenitor growth and fate resulting in fibrocystic kidney disease.

Cheng T, Agwu C, Shim K, Wang B, Jain S, Mahjoub MR
Development (Cambridge, England) 2023 Dec 15;150(24)

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The novel role of etoposide in inhibiting the migration and proliferation of small cell lung cancer and breast cancer via targeting Daam1.

Yu X, Xu T, Su B, Zhou J, Xu B, Zhang Y, Zhu Y, Jiang N, He Z
Biochemical pharmacology 2023 Apr;210:115468

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CBX7, a Potential Prognostic Biomarker in Lung Adenocarcinoma.

Yang Y, Hu Z, Sun H, Yu Q, Yang L, Yin F, Sun Y, Pu L, Zhu X, Li S, Chen X, Zhao Y
OncoTargets and therapy 2021;14:5477-5492

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Daam1 Overexpression Promotes Gastric Cancer Progression and Regulates ERK and AKT Signaling Pathways.

Zhang Y, Bai X, Zhang Y, Li Y
OncoTargets and therapy 2021;14:4609-4619

---

Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.

Hao L, Liu Y, Yu X, Zhu Y, Zhu Y
Cell proliferation 2021 Mar;54(3):e12994

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The Wnt/PCP formin Daam1 drives cell-cell adhesion during nephron development.

Krneta-Stankic V, Corkins ME, Paulucci-Holthauzen A, Kloc M, Gladden AB, Miller RK
Cell reports 2021 Jul 6;36(1):109340

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YWHAZ interacts with DAAM1 to promote cell migration in breast cancer.

Mei J, Liu Y, Yu X, Hao L, Ma T, Zhan Q, Zhang Y, Zhu Y
Cell death discovery 2021 Aug 27;7(1):221

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Overexpressed DAAM1 correlates with metastasis and predicts poor prognosis in breast cancer.

Mei J, Xu B, Hao L, Xiao Z, Liu Y, Yan T, Zhu Y
Pathology, research and practice 2020 Mar;216(3):152736

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Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.

He QJ, Wang P, Liu QQ, Wu QG, Li YF, Wang J, Lee SC
American journal of physiology. Cell physiology 2020 Jan 1;318(1):C48-C62

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A DAAM1 3'-UTR SNP mutation regulates breast cancer metastasis through affecting miR-208a-5p-DAAM1-RhoA axis.

Mei J, Yan T, Huang Y, Xia T, Chang F, Shen S, Hao L, Chen Y, Wang Z, Jiang X, Xu B, Zhu Y
Cancer cell international 2019;19:55

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Divergent roles of the Wnt/PCP Formin Daam1 in renal ciliogenesis.

Corkins ME, Krneta-Stankic V, Kloc M, McCrea PD, Gladden AB, Miller RK
PloS one 2019;14(8):e0221698

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DAAM1-mediated migration and invasion of ovarian cancer cells are suppressed by miR-208a-5p.

Mei J, Huang Y, Hao L, Liu Y, Yan T, Qiu T, Xu R, Xu B, Xiao Z, Jiang X, Hu K, Zhu Y
Pathology, research and practice 2019 Jul;215(7):152452

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Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1.

Williams SK, Weiner ZP, Gilmore RD
PloS one 2018;13(5):e0197413

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Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.

Rana MK, Aloisio FM, Choi C, Barber DL
Molecular biology of the cell 2018 Jun 15;29(12):1465-1475

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miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer.

Xiong H, Yan T, Zhang W, Shi F, Jiang X, Wang X, Li S, Chen Y, Chen C, Zhu Y
Cellular signalling 2018 Apr;44:33-42

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Implication of overexpression of dishevelled-associated activator of morphogenesis 1 (Daam-1) for the pathogenesis of human Idiopathic Pulmonary Arterial Hypertension (IPAH).

Yanai S, Wakayama M, Nakayama H, Shinozaki M, Tsukuma H, Tochigi N, Nemoto T, Saji T, Shibuya K
Diagnostic pathology 2017 Mar 14;12(1):25

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DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility.

Nishimura T, Ito S, Saito H, Hiver S, Shigetomi K, Ikenouchi J, Takeichi M
The Journal of cell biology 2016 Nov 21;215(4):559-573

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Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis.

Li D, Hallett MA, Zhu W, Rubart M, Liu Y, Yang Z, Chen H, Haneline LS, Chan RJ, Schwartz RJ, Field LJ, Atkinson SJ, Shou W
Development (Cambridge, England) 2011 Jan;138(2):303-15

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human heart tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP(DAAM1 antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using DAAM1 antibody 14876-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 14876-1-AP(DAAM1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 14876-1-AP(DAAM1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue