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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB880-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL19/MIP-3 beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCL19/MIP-3 beta in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10, 11, 12, 17, 20, 21, 22, 24, 25, 27 or recombinant rat CCL20 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q548P0- Isotype
- IgG
- Antibody clone number
- 87102
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Thymic T cell development and progenitor localization depend on CCR7.
Misslitz A, Pabst O, Hintzen G, Ohl L, Kremmer E, Petrie HT, Förster R
The Journal of experimental medicine 2004 Aug 16;200(4):481-91
The Journal of experimental medicine 2004 Aug 16;200(4):481-91
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CCL19/MIP-3 beta and Neutral-ization by Mouse CCL19/MIP-3 beta Antibody. Recombinant Mouse CCL19/ MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB880). The ND50 is typically 3-15 µg/mL.
