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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunocytochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- AF880 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL19/MIP-3 beta Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant rat CCL19/MIP-3 beta is observed and less than 10% cross-reactivity with recombinant human CCL19/MIP-3 beta is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q548P0- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CCR7-CCL19/CCL21 Axis is Essential for Effective Arteriogenesis in a Murine Model of Hindlimb Ischemia.
Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice.
CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo.
Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
Nossent AY, Bastiaansen AJ, Peters EA, de Vries MR, Aref Z, Welten SM, de Jager SC, van der Pouw Kraan TC, Quax PH
Journal of the American Heart Association 2017 Mar 8;6(3)
Journal of the American Heart Association 2017 Mar 8;6(3)
Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
Ibrahim MK, Barnes JL, Osorio EY, Anstead GM, Jimenez F, Osterholzer JJ, Travi BL, Ahuja SS, White AC Jr, Melby PC
Infection and immunity 2014 Aug;82(8):3098-112
Infection and immunity 2014 Aug;82(8):3098-112
CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B.
Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS
Journal of immunology (Baltimore, Md. : 1950) 2010 May 15;184(10):5571-81
Journal of immunology (Baltimore, Md. : 1950) 2010 May 15;184(10):5571-81
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 1;182(11):6851-60
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 1;182(11):6851-60
CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice.
Demoor T, Bracke KR, Vermaelen KY, Dupont L, Joos GF, Brusselle GG
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):8186-94
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):8186-94
CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo.
Worbs T, Mempel TR, Bölter J, von Andrian UH, Förster R
The Journal of experimental medicine 2007 Mar 19;204(3):489-95
The Journal of experimental medicine 2007 Mar 19;204(3):489-95
Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
Ato M, Stäger S, Engwerda CR, Kaye PM
Nature immunology 2002 Dec;3(12):1185-91
Nature immunology 2002 Dec;3(12):1185-91
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL19/MIP-3 beta in Mouse Splenocytes. CCL19/MIP-3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL19/MIP-3 beta in Mouse Dendritic Cells. CCL19/MIP-3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL19/MIP-3 beta and Neutralization by Mouse CCL19/MIP-3 beta Antibody. Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 μg/mL.
